Crystal structure of theEscherichia coli SbmC protein that protects cells from the DNA replication inhibitor microcin B17
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چکیده
منابع مشابه
Initiation of DNA replication by the Escherichia coli dnaG protein: evidence that tertiary structure is involved.
The dnaG protein of Escherichia coli initiates DNA replication by synthesizing primer oligonucleotides for elongation by DNA polymerae. The experiments reported here probe the nature of the nucleic acid element recognized by the dnaG protein. Three well-separated groups of nucleotides within the negative-strand origin of the single-stranded phage phi K are protected by the dnaG protein against ...
متن کاملThe antibiotic microcin B17 is a DNA gyrase poison: characterisation of the mode of inhibition.
Microcin B17 is a 3.1-kDa bactericidal peptide; the putative target of this antibiotic is DNA gyrase. Microcin B17 has no detectable effect on gyrase-catalysed DNA supercoiling or relaxation activities in vitro and is unable to stabilise DNA cleavage in the absence of nucleotides. However, in the presence of ATP, or the non-hydrolysable analogue 5'-adenylyl beta,gamma-imidodiphosphate, microcin...
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Papillomaviral infection causes both benign and malignant lesions and is a necessary cause of cervical carcinoma. Replication of this virus requires the replication initiation proteins E1 and E2, which bind cooperatively at the origin of replication (ori) as an (E1)2-(E2)2-DNA complex. This is a precursor to larger E1 complexes that distort and unwind the ori. We present the crystal structure o...
متن کاملFragments of the Bacterial Toxin Microcin B17 as Gyrase Poisons
Fluoroquinolones are very important drugs in the clinical antibacterial arsenal; their success is principally due to their mode of action: the stabilisation of a gyrase-DNA intermediate (the cleavage complex), which triggers a chain of events leading to cell death. Microcin B17 (MccB17) is a modified peptide bacterial toxin that acts by a similar mode of action, but is unfortunately unsuitable ...
متن کاملCrystal structure of the PdxY Protein from Escherichia coli.
The crystal structure of Escherichia coli PdxY, the protein product of the pdxY gene, has been determined to a 2.2-A resolution. PdxY is a member of the ribokinase superfamily of enzymes and has sequence homology with pyridoxal kinases that phosphorylate pyridoxal at the C-5' hydroxyl. The protein is a homodimer with an active site on each monomer composed of residues that come exclusively from...
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ژورنال
عنوان ژورنال: Proteins: Structure, Function, and Genetics
سال: 2002
ISSN: 0887-3585,1097-0134
DOI: 10.1002/prot.10102